No, the lye amount would remain the same for each layer, you would just have extra water in that one layer, so it may be softer for longer than the other layers. How much water is used has nothing to do with how much lye is needed to saponify an oil.
If you mix up a lye solution and some water evaporates out, you now have a slightly stronger lye solution than intended. If you pour off a certain amount and use it, what is left over for the last layer will be less than you need, which means there will be less lye than you need because the concentration will be constant (or nearly so, there is still some evaporation going on the whole time). The
amount of water is completely irrelevant. The
concentration of the lye is important because that is how you determine how much lye you're adding to each layer. Assuming a water to lye ratio of 2:1, every ounce of solution is one third of an ounce of lye. But if your solution has had some water evaporated out, then each ounce of solution contains
more than a third of an ounce of lye because lye doesn't evaporate out, only the water does.
Which means, when you get to the last layer and you're an ounce short of solution, you are a third of an ounce short of lye for that layer, regardless of the amount of water present. Which is why I recommended adding the lost water back in
before beginning to mix each layer. If your recipe calls for X amount of lye solution for each layer, and your solution isn't the correct concentration , you need to make it the correct concentration before you start or X ounces of water will not have the right amount of lye for each layer.
It is possible to just weigh what you have after cooling and divide that by the number of layers you're making, true. Depending on the recipe, it could even trace faster. The amount of water isn't important, but the ratio to lye is important when using this method.